Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Anti-HTT (mouse monoclonal) | EMD Millipore | mAb5490, RRID:AB_2233522 |
WB (1:500) |
| Antibody | Anti-GFP (rabbit polyclonal) | ThermoFisher | A-11122, RRID:AB_221569 |
WB (1:1000) |
| Antibody | Anti-HTT (mouse monoclonal) | Novartis | 2B7 | HTRF (0.023 μg/mL) |
| Antibody | Anti-laminC (mouse monoclonal) | Hybridoma Bank | LC28.26, RRID:AB_528339 |
WB (1:1000) |
| Antibody | Anti-GFAP (rabbit polyclonal) | DAKO | Z0334, RRID:AB_10013382 | IF (1:500) |
| Antibody | Alpha-tubulin (rabbit polyclonal) | Abcam | EP1332Y, RRID:AB_922700 |
WB (1:1000) |
| Antibody | Anti-HTT (mouse monoclonal) | SigmaAldrich | mAb2166, RRID:AB_11213141 | HTRF (1.4 μg/mL) |
| Antibody | Anti-rabbit IgG Alexa 488 (goat polyclonal) | Invitrogen | A-11008, RRID:AB_143165 |
IF (1:500) |
| Antibody | Anti-Serpina1a (rabbit polyclonal) | Invitrogen | PA5-16661, RRID:AB_10985745 | IF (1:250) |
| Antibody | RDye 680RD anti-Rabbit IgG (goat polyclonal) | LI-COR Biosciences | 925-68071, RRID:AB_2721181 |
WB (1:5000) |
| Antibody | IRDye 800CW anti-Mouse IgG (goat polyclonal) | LI-COR Biosciences | 925-32210, RRID:AB_2687825 |
WB (1:5000) |
| Chemical compound, drug | Lipofectamine 2000 | Life Technologies | 11668 | |
| Chemical compound, drug | EDTA-free protease inhibitor | Calbiochem | 539134 | |
| Commercial assay, kit | miRNeasy Mini Kit | Qiagen | 217004 | |
| Commercial assay, kit | Illumina TruSeq Stranded mRNA | Illumina | 20020595 | |
| Commercial assay, kit | Tyramide-Cy3 Plus kit | Perkin Elmer | NEL744001KT | |
| Commercial assay, kit | PicoGreen | Quant-iT PicoGreen dsDNA Assay Kit | P7589 | |
| Cell line (Mus musculus) | STHdhQ111/Q7 Cells | Coriell Cell Repositories | CH00096 | |
| Strain, strain background (Drosophila) | White mutant, background genotype | Bloomington Drosophila Stock Center | 3605 | w1118 |
| Genetic reagent (Drosophila) | Non-targeting hpRNA | Vienna Drosophila Resource Center | 13974 | |
| Strain, strain background (Drosophila) | repo-Gal4 | Bloomington Drosophila Stock Center | 7415 | w1118; P{w+m*=GAL4}repo/TM3, Sb1 |
| Strain, strain background (Drosophila) | elav-Gal4 | Bloomington Drosophila Stock Center | 458 | P{GawB}elavC155 |
| Genetic reagent (Drosophila) | N-terminal HD model | Botas Laboratory | Branco et al., 2008 | UAS-HTTNT231Q128/TM6B, tubulin-GAL80 (N-terminal) |
| Genetic reagent (Drosophila) | Full-length HD model | Botas Laboratory | This paper | UAS-HTTFL200Q/CyO (full-length) |
| Genetic reagent (Drosophila) | siRNA against human mutant HTT | Botas Laboratory Kaltenbach et al., 2007 |
UAS-siHTT | |
| Genetic reagent (Drosophila) | Classic CenG1A loss-of-function allele | Bloomington Drosophila Stock Center | 44301 | CenG1ALOF or y1w*;Mi{MIC}CenG1AMI06024 (Figure 3D) |
| Genetic reagent (Drosophila) | Classic vlc loss-of-function allele | Bloomington Drosophila Stock Center | 10366 | vlcLOF or y1w67c23;P{w+mc = lacW}vlck01109/CyO (Figure 3D) |
| Genetic reagent (Drosophila) | Classic trn loss-of-function allele | Bloomington Drosophila Stock Center | 4550 | trnLOF or y1w67c23;P{w+mc = lacW}trnS064117/TM3, Sb1 Ser1 (Figure 3D) |
| Genetic reagent (Drosophila) | Classic cora loss-of-function allele | Bloomington Drosophila Stock Center | 9099 | coraLOF or P{ryt7.2=neoFRT}43D cora14/CyO (Figure 3D) |
| Genetic reagent (Drosophila) | RNAi against Sytbeta | Vienna Drosophila Resource Center | 106559 | UAS-SytbetahpRNA (Figure 3D) |
| Genetic reagent (Drosophila) | RNAi against mGluR | National Institute of Genetics, Japan | 11144 R-3 | UAS-mGluRRNAi (Figure 3D) |
| Genetic reagent (Drosophila) | Neuronal mCD8::GFP reporter line | Bloomington Drosophila Stock Center | 5146 | P{w+mW.hs=GawB}elavC155, P{w+mC = UAS-mCD8::GFP.L}Ptp4E[LL4], P{ry[+t7.2]=hsFLP}1, w* |
| Genetic reagent (Drosophila) | Classical loss of function and overexpression alleles in Drosophila | Bloomington Drosophila Stock Center | Provided in Supplementary files 4 and 5 | |
| Genetic reagent (Drosophila) | RNAi alleles in Drosophila | Vienna Drosophila Resource Center | Provided in Supplementary files 4 and 5 | |
| Genetic reagent (Drosophila) | Cytological duplication alleles in Drosophila | GenetiVision | Provided in Supplementary files 4 and 5 | |
| Genetic reagent (M. musculus) | HdhzQ175 Mice | Jackson Laboratories | 027410 | B6J.129S1-Htttm1Mfc/190ChdiJ |
| Recombinant DNA reagent | pMF3 Vector | Drosophila Genome Resource Center | 1237 | |
| Software, algorithm | Adept Desktop | Omron | N/A | |
| Software, algorithm | Video Savant | IO Industries | N/A | |
| Software, algorithm | MatLab with Image Processing Toolkit and Statistics Toolkit | MathWorks | https://www.mathworks.com/products/matlab.html | |
| Software, algorithm | RSLogix | Rockewell Automation | N/A | |
| Software, algorithm | Ultraware | Rockewell Automation | N/A | |
| Software, algorithm | Assay Control | SRI International | N/A | |
| Software, algorithm | FastPhenoTrack Vision Processing | SRI International | N/A | |
| Software, algorithm | TrackingServer Data Management | SRI International | N/A | |
| Software, algorithm | ScoringServer Behavioral Scoring | SRI International | N/A | |
| Software, algorithm | Trackviewer Visual Tracking and Viewing | SRI International | N/A | |
| Software, algorithm | Illustrator CC | Adobe | https://www.adobe.com | |
| Software, algorithm | R | R Project for Statistical Computing | https://www.r-project.org/ | |
| Software, algorithm | Fiji | The Fiji Team | https://fiji.sc/ | |
| Software, algorithm | Image Studio Lite | LI-COR Biosciences | https://www.licor.com/bio/image-studio-lite/ | |
| Software, algorithm | Bowtie | Langmead and Salzberg, 2012 | http://bowtie-bio.sourceforge.net/index.shtml | |
| Software, algorithm | RSEM | Li and Dewey, 2011 | https://github.com/deweylab/RSEM | |
| Software, algorithm | DESeq2 | Love et al., 2014 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html | |
| Software, algorithm | DIOPT | Hu et al., 2011 | https://www.flyrnai.org/cgi-bin/DRSC_orthologs.pl | |
| Software, algorithm | MGI | The Mouse Genome Database | http://www.informatics.jax.org/genes.shtml | |
| Software, algorithm | STRING | Szklarczyk et al., 2015 | https://string-db.org/ | |
| Software, algorithm | InfoMap | Rosvall and Bergstrom, 2008 | https://cran.r-project.org/web/packages/igraph/index.html | |
| Software, algorithm | Cytoscape | The Cytoscape Consortium | https://cytoscape.org | |
| Transfected construct (M. musculus) | AllStars Negative Control siRNA (Scramble) | Qiagen | 1027280 | |
| Transfected construct (M. musculus) | Htt SMARTPool siRNAs | Horizon Discovery Limited | L-040632-01-0005 | 5′- GAAAUUAAGGUUCUGUUGA-3′ 5′- CCACUCACGCCAACUAUAA-3′ 5′- GAUGAAGGCUUUCGAGUCG-3′ 5′- UAACAUGGCUCAUUGUGAA-3′ |
| Transfected construct (M. musculus) | Serpina1a SMARTPool siRNAs | Horizon Discovery Limited | L-043380-01-0005 | 5′- GAAUAUAACUUGAAGACAC-3′ 5′-GGGCUGACCUCUCCGGAAU-3′ 5′- UGGUAGAUCCCACACAUAA-3′ 5′- GAAAGAUAGCUGAGGCGGU-3′ |
| Sequence-based reagent | Primers for cloning human HTT | This paper | See experimental model detail | Forward 5′-gaattcGCACCGACCAAAGAAAGAAC-3′ Reverse 5′-tctagaGGCAGAAGGTTCACCAGGTA-3′ |
| Sequence-based reagent | Primers for generating in situ probes for mouse Nrxn3 including RNA polymerase promoter sequences for T3 (forward) and T7 (reverse) | Allen Brain Atlas | https://portal.brain-map.org/ | Forward: 5′- GCGAATTAACCCTCACTAAAGGGTCCTTCCCCTTTCCTCCTAA-3′ Reverse: 5′-GCGTAATACGACTCACTATAGGGCAGGCATGCTCTGTACTCCA-3′ |