Table 4.

List of Primers Used for RT-qPCR

Gene	Accession Number	Forward	Reverse
inos	NC_051345	5ʹACATCGACCCGTCCACAGTAT3’	5ʹCAGAGGGGTAGGCTTGTCTC3’
tnfα	NC_051355	5ʹGTGGAACTGGCAGAAGAG 3’	5ʹCCATAGAACTGATGAGAGG3’
il-1β	NC_051338	5ʹGCAACTGTTCCTGAACTCAACT3’	5ʹACTTTTTGGGGTCCGTCAACT3’
ym1	NC_000069	5ʹCAGGTCTGGCAATTCTTCTGAA3’	5ʹGTCTTGCTCATGTGTGTAAGTGA3’
arg1	NC_051336	5ʹCTCCAAGCCAAAGTCCTTAGAG3’	5ʹAGGAGCTGTCATTAGGGACATC3’
fizz1	NC_051346	5ʹCCAATCCAGCTAACTATCCCTCC3’	5ʹACCCAGTAGCAGTCATCCCA3’
NLRP3	NC_051345	5ʹGCCTTGAAGAGGAGTGGATAG3’	5ʹTGGGTGTAGCGTCTGTTGAG3’
β-actin	NC_051347	5ʹACGGTCAGGTCATCACTATCG3’	5ʹGGCATAGAGGTCTTTACGGATG3’

Abbreviations: A20, tumor necrosis factor alpha-induced protein 3; CYLD, cylindromatosis; EA, electroacupuncture; CX3CL1, C-X-C motif 3 chemokine ligand 1; CNS, central nervous system; CX3CR1, CX3C chemokine receptor 1; DUB, deubiquitinase; Iba-1, ionized calcium binding adapter molecule 1; IKKβ, IkB kinase beta; IL-1β, interleukin-1 beta; LUBAC, linear ubiquitin chain assembly complex; MCAO/R, middle cerebral artery occlusion/reperfusion; NF-κB, nuclear factor-κB; NLRP3, NOD-like receptor family, pyrin domain containing 3; OGD/R, oxygen-glucose deprivation; RT-qPCR, real-time quantitative polymerase chain reaction; TNF-α, tumor necrosis factor alpha; TTC, 2,3,5-triphenyltetrazolium chloride; USP18, ubiquitin-specific protease 18; UPS4, ubiquitin-specific protease 4.