Table 2.
Antibodies Used for Western Blotting and Immunofluorescence
| Antibody | Host | Source | Catalog Number | Dilution for Staining | Dilution for Western Blot |
|---|---|---|---|---|---|
| Occludin | Rabbit polyclonal | Thermo Fisher Scientific, United States | 71–1500 | 1:100 | 1:1000 |
| Claudin5 | Mouse monoclonal | Thermo Fisher Scientific, United States | 35–2500 | 1:100 | 1:1000 |
| ZO-1 | Rabbit polyclonal | Thermo Fisher Scientific, United States | 40–2200 | 1:100 | 1:1000 |
| TNF-α | Rabbit polyclonal | Abcam, United Kingdom | ab66579 | 1:1000 | |
| IL-10 | Rabbit polyclonal | Abcam, United Kingdom | ab9969 | 1:1000 | |
| TGF-β | Mouse monoclonal | Abcam, United Kingdom | ab31013 | 1:1000 | |
| iNOS | Rabbit polyclonal | Abcam, United Kingdom | ab4999 | 1:1000 | |
| MCP-1 | Rabbit polyclonal | Abcam, United Kingdom | ab25124 | 1:1000 | |
| β-actin | Rabbit polyclonal | Cell Signaling Technology, United States | 4970S | 1:1000 | |
| Anti-Mannose | Rabbit polyclonal | Abcam, United Kingdom | ab125028 | 1:1000 | |
| CD86 | Rabbit polyclonal | Thermo Fisher Scientific, United States | MA5-32078 | 1:1000 | |
| CD31 | Mouse monoclonal | Abcam, United Kingdom | ab64543 | 1:100 | |
| IgG-HRP | Mouse monoclonal | Thermo Fisher Scientific, United States | 7076S | 1:100 | |
| IgG-HRP | Rabbit polyclonal | Thermo Fisher Scientific, United States | 7074S | 1:100 | |
| Secondary Antibody | Donkey polyclonal | Thermo Fisher Scientific, United States | A-31572 | 1:200 | |
| Secondary Antibody | Donkey polyclonal | Thermo Fisher Scientific, United States | A-21202 | 1:200 |