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. 2021 May 23;9(1):e002076. doi: 10.1136/bmjdrc-2020-002076

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© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.

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Amylase-negative acinar areas (black arrows) were observed in a subset of donors, with or without type 1 diabetes, stained with three different antibody clones against amylase ((A), clone HPA045394; (B), clone LSB12950; (C), clone AB21156) on FFPE tissue. No amylase-negative acinar areas were found using IF on frozen tissue from the same donors (D). In FFPE sections, the amylase-negative areas were found both adjacent and non-adjacent to islets (white arrows). FFPE, formalin-fixed paraffin-embedded.