Figure 2.

Principal-component analysis (PCA) on elastic fibers and collagen fibers

(A) Region of interest (ROI) for single spectra extraction defined by elastic fiber TCA component of high-resolution scans in WT, Fbln5 KO, and Fbln4^SMKO.

(B) PCA scores plot of PC-4 versus PC-5 shows a clustering between WT (blue), Fbln5^KO (red), and Fbln4^SMKO (green).

(C) Statistical analysis of PC-4 scores indicated as mean score values ± SD.

(D) ROI for single spectra extraction defined by collagen fiber TCA component of high-resolution scans in WT, Fbln5 KO, and Fbln4^SMKO.

(E) PCA scores plot of PC-3 versus PC-4 shows a clustering between WT (blue), Fbln5^KO (red), and Fbln4^SMKO (green).

(F) Statistical analysis of PC-4 scores indicated as mean score values ± SD.

Scale bars, 5 μm. L, luminal side. N ≥ 6 animals per genotype. Multiple comparison 1-way ANOVA; ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001.

See also Figure S3A and S3B and Table S2.