Figure 4.

MCR decomposed spectra and images of collagen fibers in ascending (Asc) and descending (Desc) aortas of WT, Fbln5^KO, and Fbln4^SMKO

(A) Most relevant MCR spectra in Fbln4^SMKO compared with WT and Fbln5^KO. Cc6 was identified as a specific Raman spectrum in Fbln4^SMKO (orange), and Cc1 represents the native signature of collagen fibers (red).

(B) MCR images of Cc6 and Cc1 components.

(C) Statistical analysis of relative intensities of MCR images in Cc6 and Cc1.

(D) MCR images of Cc6 and Cc1 in WT and Fbln4^SMK in ascending and descending aortas. Three representative images per genotype.

(E) Statistical analysis of relative intensities of MCR images in Cc6 and Cc1.

Scale bars, 5 μm. L, luminal side. N = 6 animals per genotype, data points represent mean intensity values of 3 images ± SD, 1-way ANOVA (Cc6) or Kruskal-Wallis test (Cc1) were performed; ∗p < 0.05, ∗∗p < 0.01.

See also Figure S3F and Tables S1 and S2.