Fig. 7. Meis function in limb-bud AP prepatterning.

a Whole-mount in situ hybridization analysis of Shh and its targets Cdon and Patched1 (Ptch1) in WT and M1HT;M2KO HLs at E10. Limbs analyzed: N = 4, N = 4, and N = 2, respectively. Whole-mount in situ hybridization analysis of the expression of Hand2 (b) (N = 6 FLs and N = 6 HLs), Gli3 (c) (N = 2 FLs and N = 2 HLs), Hoxd13 (d) (N = 8 FLs and N = 8 HLs) and Hoxd9 (e, upper panels) (N = 4 FLs) at E10.5 in WT and M1KO;M2KO FLs and HLs. Scale bars = 100 μm. (e lower panels) HOXD9 immunofluorescence in E10.5 FLs (N = 3 FLs). Scale bar = 100 μm. f Map of the Hand2 locus with its regulatory regions and ChIP-seq signal for Meis and HOXD13⁶⁴. The position of the mm1689 enhancer (gray bar), the associated Meis and HOXD13-binding sites (blue peaks and green bar, respectively), and the 3 kb region deleted (orange bar) are shown in a zoomed region. g Whole-mount in situ hybridization analysis of Hand2 at E10 in embryos carrying the 3 kb regulatory region deletion (N = 4 homozygous FLs and HLs and N = 4 heterozygous FLs and HLs). Arrowheads point to protein or gene expression downregulation. Source images are available at Mendeley Data⁸⁰ [https://data.mendeley.com/datasets/r774bxyf8d/2].