FIGURE 2.

DNA gyrase in Francisella, showing critical residues. The Francisella tularensis tularensis ScuS4 GyrA (A), GyrB (B), and ParE (C) amino acid sequences were used in conjugation with SwissModel (Schwede et al., 2003) to generate three-dimensional protein models. The model of each with the highest global model quality estimation score is presented here in orange. On each model, mutations leading to enhanced resistance were mapped. Blue indicates a substitution of residue, red a deletion, green an insertion after the indicated residue, and magenta either a deletion or a substitution at that residue. Protein models were built in SWISS-MODEL and illustrated in Geneious (Schwede et al., 2003; Kearse et al., 2012).