Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 25;11:10931. doi: 10.1038/s41598-021-90263-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Anti-fibrosis effects of hit compounds in phenomic-based 2D culture of hepatic stellate cells. (A) Intensity of α-SMA and F-actin in hepatic stellate cells in a 2D culture system treated with TGF-β1. Cells were treated with 2.5, 5, 10, or 20 ng/ml TGF-β1 in 2% FBS media. (B) Calculated intensity of α-SMA and F-actin when LX2 cells were treated with TGF-β1 concentration. (C) The quantitative graph of mesenchymal-related proteins (α-SMA and FAP) and ECM-related protein (collagen 1) expressions depending on treating with TGF-β1 concentration in LX2 cells. (D) Representative images of α-SMA treated 10 µM pirfenidone, 0.5 µM or 1 µM retinoic acid and forskolin with 20 ng/ml TGF-β1 for 48 h in LX2 cells. (E) Expression of EMT-related protein (N-cadherin, E-cadherin, Snail) when LX2 cells were treated with 0.5 or 1 µM of retinoic acid or forskolin with or without 20 ng/ml TGF-β1 for 48 h by western blot assay. (F) The quantitative of western blot images was analyzed. All images were obtained using the Operetta CLS system. Data are expressed as means ± SD (n = 3). ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 compared to the control group, ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared to the TGF-β1 treatment group.