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| 10× RT BufferA | 2.5 |
| 25 mM each dNTP | 0.5 |
| 1 M DTT | 0.1 |
| RT primer mixB | 2.0 |
| Ribonuclease inhibitor | 0.5 |
| Reverse transcriptase | 0.5 |
| Water | 13.8 |