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. 2021 May 25;11:10910. doi: 10.1038/s41598-021-90060-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of APS supplementation on gut development, intestinal mucosa enzyme activity and tight junction-related protein mRNA expression in pair-fed vs. S. ser. Enteritidis-challenged groups. (A) Hematoxylin and eosin (HE) staining of the duodenum and jejunum on days 21 and 42 in the four experimental groups (n = 6). The villus and crypts from each segment were measured with a light microscope equipped with Image-Pro Plus software (version 6.0, Motic Images software, Motic China Group Co., Ltd., Xiamen, China, https://www.semi.org/en/resources/member-directory/motic-china-group-co-ltd) and stained with HE, × 100 magnification, (B) The diamine oxidase (DAO) activity in the duodenal and jejunal mucosa and mRNA expression of tight junction-related proteins in the jejunum, including claudin-1, occludin, and MUC-2 (n = 6).