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. 2021 May 25;11:10910. doi: 10.1038/s41598-021-90060-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Correlations between significantly modified microbes (richness > 0.5%) and health parameters of broilers were analyzed by using Spearman’s correlation in SPSS Statistics 23.0 with the Bivariate correlation analysis and visualized with the heatmap (Heml 1.0.3.7, heatmap illustrator, http://hemi.biocuckoo.org/down.php). *P < 0.05, **P < 0.01 (Spearman’s correlation analysis). ADG, average daily gain; ADFI, average daily feed intake; F/G, ratio of ADFI to ADG; BW, body weight; IgG, Immunoglobulin G; IgA, Immunoglobulin A; sIgA, secretory IgA; sIgG, secretory IgG; D-IL-6, duodenal interleukin 6; D-IL-8, duodenal interleukin 8; D-TNF-α, duodenal tumor necrosis factor-α; J-IL-6, jejunal interleukin 6; J-IL-8, jejunal interleukin 8; J-TNF-α, jejunal tumor necrosis factor-α; I-IL-6, ileal interleukin 6; I-IL-8, ileal interleukin 8; I-TNF-α, ileal tumor necrosis factor-α; D-DAO, duodenal diamine oxidase; J-DAO, jejunal diamine oxidase.