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. 2020 Sep 16;42(6):861–870. doi: 10.1038/s41401-020-00521-3

Fig. 1. Differentiation and characterization of striatal GABA neurons.

Fig. 1

a Schematic overview of differentiation strategies involving a four developmental stage protocol used to obtain striatal MSNs. b Quantitative assessment of dendritic development in terms of total dendritic length and dendrite number after cell plating (n = 10). c Immunofluorescence images of markers of various stages of differentiation. TUJ1, DARPP32, GABA, and GAD65/67 were used to stain striatal GABA neurons, and DAPI was used to stain the nucleus. Scale bar = 50 μm. d Quantification of the expression of markers of various differentiation stages. Efficiencies are presented as the percentage of positive cells ± SEM in all fields counted (n = 3). EB embryoid body, NE neurosphere, SHH sonic hedgehog, PUR purmorphamine, MSNs medium spiny neurons.