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. 2020 Sep 16;42(6):861–870. doi: 10.1038/s41401-020-00521-3

Fig. 6. Lasting effect of a single dose of ketamine on dendritic growth in MSNs.

Fig. 6

a Schematic and timeline of the experiments. Morphological analyses were performed on days 35, 40, and 45 after exposure to ketamine. b Comparison of MSN morphology between the vehicle- and ketamine-treated groups. The box shows magnified and traced neurons in each group. Scale bar = 50 μm. c–e Morphometric assessment of MSNs was performed on day 35 (c), day 40 (d), and day 45 (e) after ketamine treatment. Two structural plasticity parameters were measured, namely, total dendritic length, and dendrite number. f Cultured MSNs treated with 100 μM ketamine or vehicle for 24 h were subjected to Western blotting with antibodies against total HDAC6, acetyl-α-tubulin and α-tubulin at different time points. β-Actin was used as an internal reference. g The expression of total HDAC6 and acetyl-α-tubulin was calculated (n = 3). h Cultured MSNs treated with 100 μM ketamine or vehicle for 24 h were subjected to Western blotting with antibodies against HDAC6. α-Tubulin was used as a control for cytoplasmic proteins, while H3 was used as a control for nuclear proteins. i The expression of HDAC6 in the cytosol and nucleus was calculated. The data are expressed as the mean ± SEM (n = 3); *P < 0.05 vs. the vehicle group.