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. 2021 May 25;11:10925. doi: 10.1038/s41598-021-90465-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mitoplast patch-clamp measurement and single-channel recordings of channels observed after transient transfection with the BK_Ca VEDEC-encoding plasmid. (A) Schematic depiction of the patch-clamp channel measurement of the inner mitochondrial membrane: mitoplast preparation and single-channel patches in the inside-out configuration. (B) Representative recording of single- and (C) multiple-channel activity in the patch of the inner mitochondrial membranes after transient transfection of HEK293T cells with the BK_Ca VEDEC-encoding plasmid. Single- and six-mitoBK_Ca channel activities are presented. Recordings were performed at holding potential + 40 mV.