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. 2021 May 25;11:10925. doi: 10.1038/s41598-021-90465-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the presence of the BK_Ca α subunit in wild-type and VEDEC transiently transfected HEK293T cells. (A) Analysis of selected gene expression using qualitative PCR. (B) Real-time PCR analysis of the expression levels of selected genes. (C) Western blot analysis of the BK_Ca pore-forming subunit in the mitochondrial fraction of wild-type and transfected cells. (D) Localization of VEDEC in HEK293T cells after transient transfection. Confocal images of cultured cells stained with MitoRed as a mitochondrial marker (upper panel, red channel) and an anti-PDI antibody as an endoplasmic reticulum marker (lower panel, red channel). The pore-forming α subunit was stained with an anti-BK α antibody (green channel). The superimposition of the two signals revealed the partial mitochondrial and ER localization of the BK_Ca α subunit (orange).