Fig. 3. NI301A binds small and large ATTRwt aggregates extracted from patient tissues.

Semi-native SDS-PAGE analysis of the amyloid fibril extracts prepared from frozen post-mortem cardiac tissues presenting TTR amyloid (ATTR+ samples, #1–4), no amyloid (A− samples, #5–8), or non-TTR amyloid (A+TTR− sample, # 9). Identical SDS-PAGE gels were processed for western blot analysis with the antibodies NI301A, 39-44, and Dako A0002, or stained with the silver method. The low ATTR staining intensity of sample 4 is consistent with the low amount of TTR amyloid observed by IHC in this sample.