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. 2020 Sep 23;42(6):909–920. doi: 10.1038/s41401-020-00526-y

Fig. 2. Histological analyses of heart tissues.

Fig. 2

Adult Tg-mtCAST/tTA (TG) mice and their wild-type (WT) littermates were injected with STZ (50 mg· kg−1 ·d−1, i.p.) for five consecutive days. Two months after STZ injection, heart tissues were collected and processed for histological analyses. a A representative histological picture of fluorescence-conjugated wheat germ agglutinin staining (red). b Quantitation of the cardiomyocyte cross-sectional area. c Real-time RT-PCR analysis of ANP mRNA expression in heart tissues. d A representative histological picture of Sirius red staining (red). e Quantitation of collagen deposition. f Real-time RT-PCR analysis of collagen-III mRNA expression in heart tissues. The data are presented as the mean ± SD of six different heart tissues from each group. *P < 0.05 vs WT+sham and #P < 0.05 vs WT+STZ.