Table 1.
Cryo-EM structure determination and model statistics.
| IP3R1-ND | IP3R1-LMNG | |
|---|---|---|
| EMDB-23337 | EMDB-23338 | |
| PDB-7LHE | PDB-7LHF | |
| Data collection | ||
| Microscope | Titan Krios G3 | Titan Krios G3 |
| Voltage (kV) | 300 | 300 |
| Detector | Gatan K2 Summit | Gatan K2 Summit |
| GIF energy filter (eV) | 20 | 20 |
| Magnification | 130,000 | 130,000 |
| Pixel size (Å) | 1.07 (0.535) | 1.07 (0.535) |
| Total dose (e/Å2) | 56 | 42 |
| Dose rate (electrons/Å2/s) | 8 | 6 |
| Exposure time (sec) | 7 | 7 |
| Defocus range (μm) | −0.8 to −3.5 | −0.8 to −3.5 |
| Movie stacks | 22,000 | 19,105 |
| Subframes | 35 | 35 |
| Data processing | ||
| Defocus determining software | CTFFIND3 | GCTF |
| Motion correction software | Motioncorr2 | Motioncorr2 |
| Refine software | cryoSPARC | RELION 3.0 |
| Particle picking script | Template picker | e2boxer.py |
| Number of boxed particles | 4,801,610 | 1,407,714 |
| Number of particles after 2D classification | 1,495,402 | 1,011,190 |
| Number of particles in final reconstruction | 573,723 | 303,481 |
| Symmetry imposed | C4 | C4 |
| Map resolution (Å) | 3.3 | 2.96 |
| Atomic model | ||
| Ramachandran outliers (%) | 0.25 | 0.08 |
| Ramachandran favored (%) | 88.91 | 89.65 |
| Rotamer outliers (%) | 0 | 0.05 |
| C-beta deviations | 0 | 4 |
| RMS (bonds) | 0.0044 | 0.0045 |
| RMS (angles) | 1.04 | 1.05 |
| Molprobity score | 1.92 | 1.91 |
| Molprobity clashscore | 6.21 | 6.36 |