FIGURE 2.

Schematic diagram of differentiation of hPSCs into cardiomyocytes. (A) Schematic diagram of matrix sandwich protocol. Extracellular matrix application promoted epithelial–mesenchymal transition of human PSCs. (B) Schematic diagram of GiWi small molecule differentiation protocol, which proved that timing regulation of Wnt signal was critical. (C) Schematic diagram of activin-A/BMP-4/VEGF protocol, which efficiently differentiated cardiomyocytes from both integrated and non-integrated hiPSCs. (D) Schematic diagram of flexible Wnt signal suppression protocol, which indicated that the differentiation of various cell types can be flexibly changed.