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. 2021 May 12;12:641420. doi: 10.3389/fpls.2021.641420

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Copyright © 2021 Reyes-Barrera, Soria-Guerra, López-Martínez, Huerta, Salinas-Jazmín, Cabello-Gutiérrez and Alpuche-Solís.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Components of the transformation vector for EB expression in Chlamydomonas reinhardtii. EB gene sequence optimized for expression in Chlamydomonas reinhardtii cloned between KpnI and XbaI restriction sites into pChlamy_1 vector. The EB peptide amino acid sequence has additional components: Signal peptide: Pr1a sequence; EK site: Enterokinase protease site; 6his: six-histidine tag; KDEL: reticulum endoplasmic retention tag. The pChlamy vector has the following elements: Hsp70A-RbcS2 promoter, Int-RbcS2 is RuBisCO Small Subunit 2 intron, B2 tubulin promoter, and Aph7 gene from hygromycin resistance.