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PCR assay for the detection of EB peptide gene in nucleus transformed microalgae. Amplification of an expected 116-bp product corresponding to the EB gene of nuclear transgenic lines (a, b, f), (Agr): Positive control EB-pChlamy_1 purified from Agrobacterium tumefaciens; (+): Positive control EB-pChlamy_1 purified from E. coli; (−): negative control; (WT): wild type strain DNA; (M) DNA molecular weight marker (100 bp ladder).
