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. 2021 May 12;9:652607. doi: 10.3389/fcell.2021.652607

FIGURE 3.

FIGURE 3

The oxygen sensor, JMJD6, is required for fibronectin matrix formation. (A) Western blot and corresponding densitometry for FN in the first trimester (6–8 weeks, 9–12 weeks, and TC pMSCs). n = 3 separate pMSC isolations. (B) (left panel) Western blot and densitometry for JMJD6 and FN in TC pMSCs upon JMJD6 siRNA knockdown (siJMJD6). n = 3 separate pMSC isolations. (B) (right panel) IF for FN (green) and JMJD6 (red) following siJMJD6 in TC pMSCs. Nuclei were counter-stained with DAPI. Scale bars at 63 × magnification represent 7 μM. Representative of three separate pMSC isolations. (C) Western blot and corresponding densitometry for FN and JMJD6 upon JMJD6 siRNA knockdown (siJMJD6) in concert with NH4Cl exposure of TC pMSCs. n = 3 separate pMSC isolations. (D) Western blots and associated densitometry for JMJD6 and FN in TC pMSCs overexpressing (OE) WT JMJD6 (left panel) or mutated JMJD6 constructs (Mut JmjC; right panel). EV, vehicle control. n = 3 separate pMSC isolations, Actin (ACTB) served as loading control. Data are presented as mean ± SEM. *P ≤ 0.05 **P ≤ 0.01 (unpaired Student’s t-test; one-way ANOVA and Bonferroni post-test).