TABLE 1.
Summary of single-cell RNA-Seq analyses performed in the adult mammalian heart.
| Heart sample | Cell target | Strain | Sex | Condition | Injury stage | Platform | Cell/Nuclei sequenced number | Single-cell or nuclei | References |
| Complete | Cardiac cells | C57BL/6NRj, Fzt:DU | Male | Normal | – | 10X Genomics | 11,672 | Nuclei | Galow et al., 2020 |
| Ventricles | Cardiac cells | C57BL/6J | Not specified | Normal and ischemia reperfusion | 3 dpi | SORT-seq | 932 | Cell | Gladka et al., 2018 |
| Ventricles | Cardiac cells | Mixed C57BL/6J | Mixed | Neonatal, Normal and MI (LAD) | 3, 7, and 14 dpi | SORT-seq | 1,939 | Cell | Kretzschmar et al., 2018 |
| Atria and ventricle | Cardiac cells | Human | Female and male | Normal | – | 10X Genomics | 45,870/363,213 | Cell and nuclei | Litviňuková et al., 2020 |
| Complete | Cardiac cells | Human | Mixed | Normal, HF and recovery | – | ICELL8 | 12,266 (normal)/5,933 (HF) | Cell | Wang et al., 2020 |
| Ventricles | Cardiac cells | C57BL/6J | Mixed | Normal and hypertension AngII-induced | 14 dpi | 10X Genomics | 29,558 | Cell and nuclei | McLellan et al., 2020 |
| Not specified | Cardiac cells | C57BL/6J | Mixed | Normal | – | 10X Genomics | >4,000 | Cell | Schaum et al., 2018 |
| Atria and ventricle | Cardiac cells | Human | Mixed | MI | – | 10X Genomics | 287,269 | Nuclei | Tucker et al., 2020 |
| Complete | Cardiac cells | Fzt:DU | Male | Normal | – | 10X Genomics | 8,635 | Nuclei | Wolfien et al., 2020a |
| LV | CMs | C57BL/6J/Human | Male | TAC/Normal and dilated cardiomyopathy | 8 weeks post-TAC/End stage | Fluidigm C1 | 359/116 | Nuclei | See et al., 2017 |
| Ventricles | CMs | C57BL/6J | Male | Normal and TAC | 8 weeks post-infarction | ICELL8 | 586 | Cell | Yekelchyk et al., 2019 |
| Complete | ECs | C57BL/6J | Male | Normal | – | 10X Genomics | 4,612 | Cell | Kalucka et al., 2020 |
| Ventricles | ECs | Mixed C57BL/6J | Mixed | Normal and MI (LAD) | 7 dpi | 10X Genomics | 3,200-4,000 | Cell | Li et al., 2019 |
| LV | Interstitial cells | 129S4/SvJaeSor | Male | Normal and MI (LAD) | 3 and 7 dpi | 10X Genomics | >30,000 | Cell | Farbehi et al., 2019 |
| Ventricles | Interstitial cells | C57BL/6J and 129S1/SvlmJ | Male | Normal and MI (LAD) | 1, 3, 5, 7, 14, and 28 dpi | 10X Genomics | 36,847 | Cell | Forte et al., 2020 |
| Ventricles | Interstitial cells | C57BL/6J | Female and male | Normal | – | 10X Genomics | 12,000 | Cell | Skelly et al., 2018 |
| Not specified | CFs and mural cells | C57BL/6J | Male | Normal | – | Smart-Seq2 | < 6,158 | Cell | Muhl et al., 2020 |
| Ventricles | CFs | C57BL/6J | Not specified | Normal and MI (LAD) | 7, 14, and 30 dpi | 10X Genomics | 32,669 | Cell | Ruiz-Villalba et al., 2020 |
| Complete | Immune cells | C57BL/6J | Female | Normal | – | 10X Genomics | 17,500 | Cell | Adamo et al., 2020b |
| Complete | Immune cells | C57BL/6J | Not specified | Normal and MI | 11 dpi | 10X Genomics | 1,780 (normal)/6,503 (MI) | Cell | Dick et al., 2019 |
| Complete | Immune cells | C57BL/6J | Male | Normal and heart failure (TAC) | 1- and 4-weeks post-infarction | 10X Genomics | >17,853 | Cell | Martini et al., 2019 |
LV, left ventricle; CMs, cardiomyocyte; ECs, endothelial cell; CFs, cardiac fibroblast; MI, myocardial infarction; TAC, transverse aortic constriction; LAD, left anterior descending artery; HF, heart failure. “ – “: means no data; “dpi” means days post-infarction; “complete” indicates whole heart analysis; “cardiac cells” means the main heart cell types; “interstitial cells” do not include cardiomyocytes and “atria and ventricle” indicate separated analysis.