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. 2021 May 25;12(6):540. doi: 10.1038/s41419-021-03831-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a TUNEL staining in newborn ovaries transfected with Xist-pcDNA3.1 (Xist OE) or the empty vector control (NC). The nucleus was stained by DAPI (blue). Scale bars: 20 μm. b Immunofluorescence staining of LC3B (green), and DDX4 (red) in newborn ovaries transfected with Xist-OE compared to NC control. Scale bars: 5 μm. Arrows indicating LC3B puncta. c WB analysis of active LC3B expression in newborn ovaries treated under indicated condition. d TEM analysis of autophagosomes in newborn ovaries transfected with Xist-OE compared to NC control. Red arrow indicating autophagosomes. Scale bars: 1 μm. e Representative images of DDX4 immunofluorescence staining (left) and quantification of follicles (right) in newborn ovaries treated under indicated condition. The nucleus was stained by DAPI (blue). Scale bars: 50 μm. f, g Representative images (f) and quantification of follicles (g) in the ovary transfected under indicated condition. Scale bar, 20 μm. Student’s t-test: *P < 0.05.