Figure 5.

Heme suppresses inflammation independently of iron levels (A) Heatmap displaying color-coded mRNA relative expression of proinflammatory and anti-oxidative genes in BMDMs treated with the noniron porphyrin SnMP (top) or MnPP (bottom) for 4 h before stimulation with LPS (10 ng/mL) for an additional 4 h. The data represent the mean expression values from three independent experiments (yellow=low expression, red=high expression). (B) Plasma ALT and AST concentrations in C57BL/6J mice treated with or without SnMP-albumin (70 μmol/kg, i.p.) and after 4 h with anti-CD40 antibody for 30 h (20 mg/kg) (n = 3). From these data, we could conclude that metalloporphyrins linked to albumin could replicate the inflammatory suppression of heme-albumin in vitro and in vivo. ****p < 0.0001, ***p < 0.001 for all panels.