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. 2021 May 25;12:3104. doi: 10.1038/s41467-021-23372-w

Fig. 5. Kindlin localization in the integrin signaling layer inside FAs depends on the PH domain.

Fig. 5

Top panel: Epifluorescence images of RFP-paxillin showing the localization of FAs. Bottom panel: 3D super-resolution images by DONALD displaying the axial localization of kindlin-2-WT (a), kindlin-2-QW (b), kindlin-2-ΔPH (c), paxillin (d) and kindlin-2-ΔPH-CAAX (e); scale bar, 3 µm. For each pixel, the average axial localization of detected single molecules is color-coded. f Distributions of the single molecule axial localizations of kindlin-2-WT (blue) and paxillin (brown) inside (top) and outside FAs (bottom). Values represent the average of the distributions obtained from different cells. Positions of occurrence maxima (zpeak) were estimated by Gaussian fitting. g Same as f, but with kindlin-2-WT (blue), kindlin-2-QW (red), kindlin-2-ΔPH (orange) and kindlin-2-ΔPH-CAAX (green). h Box plots displaying the median (notch) and mean (square) ± percentile (25–75%) of single molecule localizations inside (top) and outside FAs (bottom). Micrographs (a–h) correspond to a single experiment, but are representative of kindlin-2-WT: 6 cells, kindlin-2-QW: 9 cells, kindlin-2-ΔPH: 7 cells, paxillin: 6 cells, kindlin-2-ΔPH-CAAX: 7 cells. Where indicated, statistical significance was obtained using two‐tailed, non‐parametric Mann–Whitney rank sum test. The different conditions were compared to the mEos2‐kindlin-2-WT condition. The exact P values are indicated on the figure except when P < 0.0001. Source data are provided as a Source Data file.