Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 25;12:3104. doi: 10.1038/s41467-021-23372-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Top panel: TIRF images of photoconverted mEos2 coupled to kindlin-2-WT (a), kindlin-2-QW (b), kindlin-2-ΔPH (c), kindlin-2-ΔPH-CAAX (d), or photoconverted mEos2 alone (e). Bottom panel: TIRF images of GFP-paxillin showing the localization of FAs. Scale bar, 2 µm. f Enrichment inside FAs defined as the ratio of the average fluorescence intensity inside FAs to average fluorescence intensity outside FAs. Black bars represent medians and interquartile ranges. Displayed results correspond to pooled data from three independent experiments (kindlin-2-WT: 36 cells, kindlin-2-QW: 24 cells, kindlin-2-ΔPH: 27 cells, kindlin-2-ΔPH-CAAX: 50 cells, kindlin-2-L357A: 34 cells, kindlin-2-K390A: 30 cells, mEos2: 38 cells). Where indicated, statistical significance was obtained using two‐tailed, non‐parametric Mann–Whitney rank sum test. The different conditions were compared to the mEos2‐kindlin-2-WT condition. Otherwise, a black line indicates which conditions were compared. The exact P values are indicated on the figure except when P < 0.0001. Source data are provided as a Source Data file.