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. 2021 May 25;11:10880. doi: 10.1038/s41598-021-90087-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Signature proteotypic peptides are marked in bold, a peptide position in the HSA protein sequence in brackets. Compared types of SIL-Ex peptides are shown in different colors: SIL-TCT (blue), SIL-ExC5 (orange), SIL-ExC3N3 (green), SIL-ExC5N5 (purple). SIL-ExC5, SIL-ExC3N3, and SIL-ExC5N5 denote signature peptides extended with the natural sequence of amino acids corresponding to the target protein sequence. SIL-TCT represent signature peptides extended with a tetrapeptide (SAnYG, nY = nitrotyrosine) tag.