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. 2021 May 25;11:10880. doi: 10.1038/s41598-021-90087-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The quantitative performance depends on the reconstitution solvent for SIL-TCT (black) and SIL-ExC5 (grey) peptides. The reconstitution solvent was water with 5% ACN (A) and AmBic/SDC buffer with 5% ACN (B). The reference HSA protein concentration marked with the red line at 500 nM (determined by BCG assay and protein assay using SIL-HSA).