Table 1.
The comparison of the quantitative performance of SIL-HSA and various types of SIL-Ex peptides (SIL-Ex were reconstituted in AmBic/SDC buffer with 5% ACN except for SIL-ExC5N5 indicating optimal quantitative performance in AmBic/SDC buffer with 50% ACN).
| Peptide | SIL-TCT | SIL-ExC5 | SIL-ExC3N3 | SIL-ExC5N5 | SIL-HSA | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| nM | CV (%) | nM | CV (%) | nM | CV (%) | nM | CV (%) | nM | CV (%) | |
| DDNPNLPR | 82.50 | 14.05 | 71.61 | 3.41 | 589.77 | 7.39 | 223.41 | 9.07 | 431.64 | 8.33 |
| SLHTLFGDK | 188.88 | 10.21 | 361.20 | 8.52 | 400.10 | 11.23 | 347.96 | 0.95 | 473.12 | 5.10 |
| YLYEIAR | 382.39 | 2.33 | 383.26 | 3.87 | 567.83 | 14.64 | 523.83 | 2.99 | 468.11 | 9.15 |
| LVNEVTEFAK | 343.12 | 2.07 | 234.24 | 11.66 | 267.93 | 4.66 | 197.66 | 5.10 | 398.73 | 10.53 |
| QTALVELVK | 345.95 | 5.45 | 320.92 | 4.59 | 453.52 | 8.01 | 413.01 | 8.13 | 457.73 | 6.81 |
| Average | 268.57 | 6.82 | 274.25 | 6.41 | 432.18 | 9.19 | 341.17 | 5.25 | 445.86 | 7.98 |