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. 2021 May 25;11:10877. doi: 10.1038/s41598-021-90338-9

Table 1.

HPLC procedure for sample peak separation.

Retention (min) Flow (ml/min) A*% B**%
0 0.2 10 90
5 0.2 90 10
8 0.2 90 10
9 0.2 10 90
13 0.2 10 90

*Acetonitrile + 0.1% Tri-fluolroacetic acid; **Water + 0.1% Tri-fluolroacetic acid.