Fig. 1. RNA-seq analysis of slow-twitch and fast-twitch muscles with myofiber-specific OSKM induction.

a Illustration of the genetic makeup for myofiber-specific OSKM inducible mouse model. For simplicity, Cre⁺ represents Acta1-Cre⁺/4F^het and Cre⁻ represents Acta1-Cre⁻/4F^het mice. b Schematic representation of the experimental design. c The fpkm of OSKM in Cre⁻ and Cre⁺ EDL muscles. n = 2 biological independent samples. d The fpkm of OSKM in Cre⁻ and Cre⁺ SOL muscles. n = 2 biological independent samples. e Principal component analysis on top 500 most variables genes of Cre⁻ and Cre⁺ EDL and SOL muscles. f Euclidean distance analysis of the transcriptome of Cre⁻ and Cre⁺ EDL muscles. g Volcano plots of DE genes in EDL muscles after 2.5- or 8.5-days Dox treatment. h Enriched GO terms in the biological process of commonly changed genes after 2.5- or 8.5-days Dox treatment.