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. 2021 May 25;12:3094. doi: 10.1038/s41467-021-23353-z

Fig. 5. OSKM induction does not change the self-renewal of SCs.

Fig. 5

a Schematic representation of the experimental design for 7 days after triple CTX injury. b Immunostaining of Pax7 and MyoD, and quantification of Pax7 cells in TA muscle sections. Scale bars = 50 µm. Representative regions are shown at higher magnification. Scale bars = 25 µm. White arrows indicate Pax7+ cells. c Immunostaining of embryonic myosin heavy chain (eMHC) and Dystrophin in TA muscle sections, and the quantification of the percentage of immature myofibers that express eMHC. Scale bars = 50 µm. d H&E staining of TA muscle sections and myofiber size distributions in TA muscle sections. Scale bars = 100 µm. e Schematic representation of the experimental design for 30 days after triple CTX injury. f Immunostaining of Pax7, BrdU, and MyoD, and quantification of Pax7 cells and percentage of BrdU+ SCs in TA muscle sections. Scale bars = 25 µm. Red Arrows indicate Pax7+MyoD−BrdU+SCs. Error bars represent mean + SD of four mice. A two-sided unpaired Student’s t-test was performed.