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. 2021 May 25;12(6):535. doi: 10.1038/s41419-021-03817-2

Fig. 5. Tet2 targets MyoG to facilitate muscle regeneration.

Fig. 5

A Methylation profile of the 5’ upstream region of MyoG gene in WT or Tet2 KO myoblasts extracted from genome-wide methylation-seq. B Bisulfite sequencing results of E1 and E2 enhancers of MyoG. C H3K4me1 and H3K27ac levels in E1 and E2 enhancer regions of MyoG (n = 3). D Scheme of the luciferase reporter constructs. E Luciferase activity of the E1 or E2 enhancer (n = 3). F Scheme of the mutated luciferase reporter constructs. Black arrows indicated the mutated CpG sites. G Luciferase activity of the E2 enhancer mutant (n = 3). H Immunofluorescence staining of MyHC in MyoG knocked down myotubes. Scale bars, 200 µm. I Quantification of the fusion index in MyoG knocked down myotubes (n = 3). J Relative mRNA expression levels of differentiation related genes in MyoG knocked down myotubes (n = 3). K Immunofluorescence staining of GFP and Phalloidin in MyoG over-expressing myotubes. Scale bars, 100 µm. L Quantification of the fusion index in MyoG over-expressing myotubes (n = 3). M Relative mRNA expression levels of differentiation related genes in MyoG over-expressing myotubes (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001.