Fig. 2. SH inhibits the JNK pathway and reduces the levels of ROS inflammation. (A) The protein expression of p-JNK/JNK, Bcl-2, Bax, and Bak was detected by Western blot. (B) The apoptosis of lung tissues measured by TUNEL staining (scale bars, 50 µm). (C) The W/D ratio of lung tissues. (D and E) The levels of ROS and MDA in lung tissues. ^*p<0.05 vs. Sham group, ^†p<0.05 vs. VILI group, ^‡p<0.05 vs. VILI+SH group. The gavage dose of SH was 40 mg/kg. SH, sodium houttuyfonate; ROS, reactive oxygen species; VILI, ventalitor-induced lung injury; W/D, wet-to-dry; MDA, malonaldehyde.