Figure 6.

Differences in HLA-DR expression and distribution in circulating monocyte subsets and in T1D compared to age-matched healthy donors (HD). (A) Gating strategy for the determination of peripheral monocyte subsets by flow cytometry with the combination of CD14, CD16 and HLA-DR. Three monocyte subsets were defined: non-classical monocytes (CD14⁺CD16⁺⁺), intermediate monocytes (CD14⁺⁺CD16⁺⁺), and classical monocytes (CD14⁺⁺CD16⁺). (B) Distribution of monocyte subsets in T1D cases compared to age-matched healthy donors (HD). (C) HLA-DR expression in different monocyte subsets of T1D cases compared to age-matched healthy donors (HD). The results were from 68 autoantibody-positive T1D individuals and 68 age-matched autoantibody-negative healthy controls. Samples from T1D individuals and healthy controls were randomly divided to each independent experiment. One biological sample (from the same healthy donor and drawn at the same time) was performed as control for the experimental reproducibility. Comparisons between T1D and healthy controls were performed by unpaired t test with Welch’s correction. A p value <0.05 was considered as significant.