Overall diversity was calculated using unweighted UniFrac by PCoA (A) and NMDS (B), indicating a separation of samples between pre-treatment and post-treatment. (C) PCoA based on the weighted UniFrac distance in NJI with treatment to assess the microbial distribution among 0, 1, 3, 6, and 12 months, indicating that samples tended to be uniform at 0 and 12 months, and samples are most heterogeneous at 1–6 months. Fecal microbiota composition at the phylum level (D) and genus level (E) between pre-treatment and post-treatment. (F) Compared with pre-treatment, two phyla were significantly increased, while two phyla were significantly decreased post-treatment (all P<0.05). (G) One genus was increased whereas one genus was decreased in pre-treatment versus post-treatment (all P<0.05). Abbreviations: M0, 0 month; M1, 1 month; M3, 3 months; M6, 6 months; M12, 12 months.