Fig. 3.

The deletion of exons 7–11 in the Nnt gene of C57BL/6J substrains. Genomic polymerase chain reaction (PCR) analysis of exons 6–12 in the Nnt gene showed the absence of PCR products from exons 7–11 in the C57BL/6J, C57BL/6JJcl, C57BL/6JJmsSlc, and C57BL/6JMs substrains. The deletion of the Nnt gene occurred in the C57BL/6J colony of the Jackson Laboratory between 1976 and 1984. The branching years of each strain from the original C57BL/6J strain are given in parentheses. PCR and gel electrophoresis procedures were performed according to the protocol described in Mekada et al. (2009) [6]. PCR product size data are from Huang et al. (2006) [117].