Fig. 4.

Intraneuronal aluminum in the temporal cortex of a 60-year-old male Colombian donor with familial Alzheimer’s disease. A) Intracellular aluminum (Al) in a neuron (orange). B) AT8 immunoreactive phosphorylated tau (pTau) located via DAB staining (brown) appearing as flame-like NFT. C). Autofluorescence (green) of the non-stained adjacent section highlighting occasional deposits of intracellular lipofuscin (yellow). D) Merging of lumogallion and brightfield channels depicting aluminum and pTau co-localized in the identical neuron. Asterisks denote magnified inserts. Magnification: X 400, scale bars: 50μm.