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. 2021 May 12;13(10):2314. doi: 10.3390/cancers13102314

Figure 2.

Figure 2

Orai3 silencing increases the sensitivity to CDDP treatment in A549 and H23 cells. (A) Orai3 and Orai1 expression in A549 compared to H23 cell line. Expression at the mRNA level of Orai3 (A-a) and Orai1 (A-b) normalized to GAPDH. Western blot showing protein expression of Orai3 (A-c) and Orai1 (A-d) with their respective quantifications (A-e) and (A-f). Values presented are the mean of three independent experiments ± SEM. *** p < 0.001, ** p ˂ 0.01, * p ˂ 0.05, Student’s t-test. (B,C) Assessment of CDDP-induced apoptosis using flow cytometry Annexin V and PI staining in A549 (B-a) and H23 (C-a) cells. Quantification of the percentage of apoptosis in each condition in A549 (B-b) and H23 (C-b) cells. Values of three independent experiments are presented as mean ± SEM. *** p < 0.001 vs. control, ns: not significant. ANOVA followed by Holm–Sidak post test.