Skip to main content
. 2021 May 12;13(10):2314. doi: 10.3390/cancers13102314

Figure 5.

Figure 5

Effect of Orai3 channel overexpression on apoptosis induced by Cisplatin in A549 cells. Transfection validation with VOrai3 via Western blot showing Orai3 protein expression (A-a). Effect of Orai3 overexpression on the calcium signal in A549 cells. SOCE was measured after endoplasmic reticulum release by 1µM TG (A-b). Quantification of SOCE by measuring the ratio of the peak amplitude over the initial (A-c). Student’s t-test, ** p < 0.01, Vempty: n = 53, VOrai3: n = 89, N = 3. Assessment of CDDP-induced apoptosis using flow cytometry Annexin V and PI staining in A549 cells transfected with Vempty and VOrai3 (B). Quantification of the percentage of apoptosis (C), ANOVA followed by Holm–Sidak post-test, *** p < 0.001 ** p < 0.01 * p < 0.05, N = 3.