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. 2021 May 12;13(10):2314. doi: 10.3390/cancers13102314

Figure 6.

Figure 6

CDDP induces expression of stem cell markers in A549 cells which was prevented upon Orai3 silencing. Relative mRNA expression of Nanog (A) and SOX-2 (B) after 2–7 days CDDP treatment with respect to GAPDH and relative protein expression (D) of Nanog (C) and SOX-2 (E) normalized to Tubulin, ANOVA followed by Dunnett post-test ** p < 0.01, *** p < 0.001, N = 3. Relative mRNA expression of Nanog (F) and SOX-2 (G) after Orai3 silencing and 48 h treatment with CDDP and the respective protein expression using Western blot (I) representing Nanog (H) and SOX-2 (J) protein expression normalized to Tubulin, ANOVA followed by Holm–Sidak post-test * p < 0.05,** p < 0.01, ***p <0.001. ns: not significant, N = 3.