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. 2021 May 12;13(10):2314. doi: 10.3390/cancers13102314

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Function of Orai3 channel in CDDP-induced CD133⁺ A549 cells in calcium entry and apoptosis. Transfection validation: protein expression of Orai3 in CD133⁺ cells transfected with siCtl and siOrai3 (A). Traces representing the SOC entry measured with the ratio F340/F380 using calcium imaging technique (B-a). Cells were exposed to 1 µM TG in the absence of extracellular Ca²⁺ which depletes the intracellular Ca²⁺ stores. Extracellular calcium concentration was then brought to 2 mM in order to induce SOCE. Quantification of the SOCE by measuring the ratio of the peak amplitude over the initial (B-b). (siCtl: n = 60, siOrai3: n = 52, N = 3, *** p < 0.001, Student’s t-test, n: number of cells, N: number of passage). Flow cytometric analysis of early, late apoptosis and necrosis in CD133⁺ cells transfected with siCtl or siOrai3 (C). Bar chart represents the percentage of total apoptosis in each condition in CD133⁺ cells (D). Student’s t-test, *** p < 0.001 ** p < 0.01, N = 3.

Function of Orai3 channel in CDDP-induced CD133⁺ A549 cells in calcium entry and apoptosis. Transfection validation: protein expression of Orai3 in CD133⁺ cells transfected with siCtl and siOrai3 (A). Traces representing the SOC entry measured with the ratio F340/F380 using calcium imaging technique (B-a). Cells were exposed to 1 µM TG in the absence of extracellular Ca²⁺ which depletes the intracellular Ca²⁺ stores. Extracellular calcium concentration was then brought to 2 mM in order to induce SOCE. Quantification of the SOCE by measuring the ratio of the peak amplitude over the initial (B-b). (siCtl: n = 60, siOrai3: n = 52, N = 3, *** p < 0.001, Student’s t-test, n: number of cells, N: number of passage). Flow cytometric analysis of early, late apoptosis and necrosis in CD133⁺ cells transfected with siCtl or siOrai3 (C). Bar chart represents the percentage of total apoptosis in each condition in CD133⁺ cells (D). Student’s t-test, *** p < 0.001 ** p < 0.01, N = 3.