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. 2021 May 12;13(10):2314. doi: 10.3390/cancers13102314

Figure 9.

Figure 9

Regulation of Orai3 and stemness markers expression in CD133+ A549 cells induced by CDDP by PI3K pathway. Representative Western blotting of P-Akt and Akt proteins in A549 cells transfected with si-Orai3 or siCtl in the presence of CDDP (A) and respective protein expression (B). Data are presented as mean of three independent experiments ± SEM, Student’s t-test, *** p < 0.001. Relative mRNA expression with respect to GAPDH of Nanog (C), SOX-2 (D), and Orai3 (E) in CD133+ cells after 48 h CDDP treatment in the absence and presence of 20 μM LY294002 inhibitor. Relative protein expression of Nanog (F) and SOX-2 (G). Data are presented as mean of three independent experiments ± SEM, ANOVA followed by Holm–Sidak post-test ** p < 0.01 ***; p < 0.001, ns: not significant.