Figure 1.

Inhibition of proliferation by LM in A549 cells. A549 cells were continuously cultured in the media containing normal 0.8 mM Mg²⁺ (NM) or 0.5 mM Mg²⁺ (LM). (A) [Mg²⁺]_i was measured using Mag-fura 2. (B) The mRNA levels of Mg²⁺ transporters were examined by real-time PCR and represented in percentage to NM. (C) Cell number was counted at the time indicated. (D) The proportion of G1, S, and G2/M was measured using Muse Cell Analyzer. n = 3–4. ** p < 0.01 and * p < 0.05 compared with NM. NS p > 0.05.