FIGURE 6.
Xenografts of cells overexpressing GS show slow tumor growth under VC treatment. BALB/c nude mice (4–5 weeks old/male) were subcutaneously (s.c.) injected with 2 × 106 PC3 cells. When the tumor sizes reached 50–80 mm3, ascorbate sodium (4 g/kg, twice a day) were intraperitoneally (i.p.) administered to the mice. After 16 days, all mice were euthanized and the tumors were analyzed. (A) Tumor volume and weight changed after treatment with VC. (B) Immunohistochemical analyses showed the expression of Ki-67 (proliferation marker) decreased in the VC treatment groups, with more obvious change in the 0.06PC3 group. All data are presented as means ± SEM, *p < 0.05, **p < 0.01, n = 3. VC, Vitamin C.