Larval weight and body nutritional composition when fed on artificial diet and food plants with different GSL contents. (A) Larval fresh weight at 4–5 days (III instar) in mg (p < 0.001, n = 80–152); Figure S2 depicts the corresponding values for dry weight. (B) Protein in % of dry weight (p = 0.001, n = 20); (C) GSH concentrations in log of nmol/mg dry weight (p < 0.001, n = 20); (D) Total soluble amino acids in nmol/mg dry weight (p < 0.001, n = 20); (E) Heatmap of the mean [nmol/mg DW] of detected soluble amino acids, with yellow to green corresponding to small values and green-blue to dark blue to high values. Data were scaled across rows, comparing amounts of these amino acids between diet treatment groups. Statistical differences for A-D were assessed using a nested ANOVA mixed affect model, letters denote statistical difference at p = 0.05 level tested with Tukey post hoc test. Means, standard deviations and statistical details for A-D and individual amino acids (E) are in Table S2. AD, artificial diet; DW, dry weight; ▪ indicate outliers in the box-and-whiskers plots.