Table 3.

RT-PCR and nested PCR reaction components.

RT-PCR		Nested PCR
Component	Final Concentration	Component	Final Concentration
2× reaction mix	1×	2× PCR buffer (Vivantis)	1×
Forward primer	0.25 µM	Forward primer	0.25 µM
Reverse primer	0.25 µM	Reverse primer	0.25 µM
SS III RT/mix platinum Taq	0.8 µL	Vivantis Taq DNA polymerase	0.2 µL
100 mM DTT	5 mM	nuclease-free water	6.8 µL
nuclease-free water	3.2 µL	1:50 diluted template	2 µL
RNA template	5 µL