Table 2.
The use of 2FTNEMCNPPE in simultaneous quantification of EP, UA and FA in an EP ampule, an FA tablet and urine samples (n = 5). The amounts of EP and FA in ampoules and tablets were found to be equal to 0.98 mg mL−1 and 1.01 mg/tablet, respectively. These outcomes show no significant difference between the results of the 2FTNEMCNPPE and the nominal value on the ampoule label and tablet label (1.00 mg mL−1 and 1.00 mg/tablet, respectively) [50].
| Sample | Spiked (µM) | Found (µM) | Recovery (%) | R.S.D. (%) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EP | UA | FA | EP | UA | EP | EP | UA | FA | EP | UA | FA | |
| EP ampoule | 0 | 0 | 0 | 9.0 | - | - | - | - | - | 2.7 | - | - |
| 2.5 | 15.0 | 17.5 | 11.4 | 15.5 | 17.1 | 99.1 | 103.3 | 97.7 | 3.2 | 1.9 | 2.8 | |
| 5.0 | 25.0 | 27.5 | 14.3 | 24.8 | 27.9 | 102.1 | 99.2 | 101.4 | 3.1 | 2.3 | 2.7 | |
| 7.5 | 35.0 | 37.5 | 17.1 | 35.1 | 37.3 | 103.6 | 100.3 | 99.5 | 1.9 | 3.3 | 2.4 | |
| 10.0 | 45.0 | 47.5 | 18.5 | 45.6 | 48.8 | 97.3 | 101.3 | 102.7 | 2.2 | 1.8 | 3.1 | |
| FA tablet | 0 | 0 | 0 | - | - | 17.0 | - | - | - | - | 3.4 | - |
| 5.0 | 17.5 | 2.5 | 5.1 | 17.1 | 19.7 | 102.7 | 97.7 | 101.0 | 2.3 | 1.9 | 3.2 | |
| 10.0 | 2.5 | 5.0 | 9.8 | 22.9 | 21.9 | 98.0 | 101.8 | 99.5 | 3.1 | 2.3 | 1.9 | |
| 15.0 | 27.5 | 7.5 | 15.1 | 27.1 | 24.3 | 100.7 | 98.5 | 99.2 | 1.7 | 2.8 | 2.7 | |
| 20.0 | 32.5 | 10.0 | 19.8 | 33.5 | 27.5 | 99.0 | 103.1 | 101.8 | 2.8 | 3.1 | 1.8 | |
| 0 | 0 | 0 | - | 10 | - | - | - | - | - | - | - | |
| 7.5 | 10.0 | 30.0 | 7.4 | 20.2 | 30.9 | 98.7 | 101.0 | 103.0 | 2.9 | 3.2 | 1.6 | |
| 12.5 | 20.0 | 40.0 | 12.7 | 29.5 | 39.1 | 101.6 | 98.3 | 97.7 | 3.4 | 2.7 | 2.6 | |
| 17.5 | 30.0 | 50.0 | 18.1 | 41.2 | 49.5 | 103.4 | 103.0 | 99.0 | 1.6 | 2.6 | 3.1 | |
| 22.5 | 40.0 | 60.0 | 22.4 | 49.8 | 61.5 | 99.5 | 99.6 | 102.4 | 2.2 | 1.8 | 2.9 | |